关键词: 环磷酰胺, 生殖损伤, 小鼠, 睾丸

Abstract: Objective To explore the model of cyclophosphamide-induced reproductive damage in male mice from two aspects of animal species and cyclophosphamide dosage. Method Healthy six-week-old male ICR mice and BALB / c mice were randomly divided into 8 groups: ICR-control, ICR-60, ICR-100, ICR-200, BALB-control, BALB-60, BALB-100 and BALB-200. ICR-control and BALB-control groups were intraperitoneally injected with normal saline once a week for 5 weeks. ICR-60 group and BALB-60 group were intraperitoneally injected cyclophosphamide 60 mg / kg every day in the first 5 days. ICR-100, BALB-100 groups and ICR-200, BALB-200 groups were intraperitoneally injected with cyclophosphamide 100 mg / kg and 200 mg / kg once a week for 5 weeks, respectively. The experiment lasted for 35 days. The mice were weighed weekly and the death of the mice was recorded. On the 36th day, the mice were sacrificed, the testes and epididymis were separated, weighed, and the testis and epididymis indexes were calculated. One side of testis was fixed, embedded and sectioned, and the testis structure was observed by HE staining. The other testis was frozen, and the activity of superoxide dismutase was measured. Result The mortality of BALB-60 and BALB-200 groups was 100%, the mortality of ICR100, ICR-200 and BALB-100 groups was high, and the mortality of ICR-60 group was 0. The testis index and epididymis index of ICR experimental groups decreased to different degrees, but only the testis index of BALB-100 group decreased. HE staining showed that spermatogenic tubules were damaged in all model groups, and the degree of damage was correlated with cyclophosphamide concentration. SOD activity of testis in ICR-60 group decreased, but SOD activity of testis in other groups did not change significantly. Conclusion ICR-60 is a more suitable model of cyclophosphamide-induced mice reproductive damage.

Key words: cyclophosphamide, reproductive damage, mice, testis